Graduate Thesis Or Dissertation

 

Effects of growth-regulating treatments on eradication of PVX and PVS in potato shoot-tip culture Public Deposited

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  • Four experiments were conducted to evaluate the effects of growth-promoting treatments on potato sprouts and plantlets grown in vitro, and to determine the relationships between growth rate and eradication of PVX and PVS through shoot-tip culture. The approach consisted of exposing plant material to potential growth-regulating treatments, selecting the more promising ones and excising the shoot-tips from associated plantlets to evaluate treatment effects on virus eradication. Treatments consisted of dark-induced etiolation and growth regulators believed to have antiviral activity. Sprouts attached to seed pieces responded to the growth-promoting effect of GA₃. However, the presence of decay microorganisms made this approach unsuitable. Nodal cuttings from isolated disinfected sprouts or from plantlets grown under aseptic conditions in vitro produced more suitable plant material since growth of decay microorganisms was minimal. Nodal cuttings from plantlets developed in vitro responded more obviously to growthregulating treatments than those from sprouts. Plantlet growth was favored by adding either 2,4-D at 0.1 and 1.0 μM, or GA₃ at 0.5 and 1.0 μM to the culture media, or by inducing etiolation with darkness. Plantlet growth was reduced when 2,4-D and BAP were added to the culture medium at concentrations of 10.0 μM and 100.0 μM, respectively, at these concentrations, callus growth, excessive branching, or both were observed. Except for dark-induced etiolation, 0.5-mm shoot-tips regenerated more plantlets in less time than 0.2-mm shoot-tips. Shoot-tips excised from plantlets treated with GA₃ at 0.5 and 1.0 μM and benomyl at 25 mg ai/l grew rapidly and survived well. Small shoot-tip size (0.2 mm) favored virus elimination, especially for PVS. Out of 25 plantlets regenerated from 0.2-mm shoot-tips, 60% were PVX-free and 48% were PVS-free while only 41.5% of 41 plantlets regenerated from 0.5-mm shoot-tips were PVX-free and 9.8% PVS-free. Compared to other alternatives for virus eradication such as thermotherapy, GA₃ at 0.5 μM and benomyl at 25 mg ai/l in the culture medium seemed to offer a good alternative because of high survival rate, short regeneration time and moderate virus eradication. The virus eradicative effect of some treatments was not clearly related to their stimulative effect on growth.
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