Induction of autoantibodies in MRL/lpr mice exposed to 2% aniline denatured low-erucic acid rapeseed oil, an oil associated with the Spanish Toxic Oil Syndrome Public Deposited

http://ir.library.oregonstate.edu/concern/graduate_thesis_or_dissertations/3x816q45n

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  • In 1981, illegal processing of rapeseed oil by a Spanish oil refinery resulted in the mass foodborne illness epidemic known as Toxic Oil Syndrome (TOS). The toxic oil associated with this epidemic was sold in neighborhood markets and by itinerant salesmen as inexpensive olive oil. Ingestion of the toxic oil resulted in more than 20,000 illnesses and over 1,500 deaths in Spain. The etiologic agent of TOS remains unknown. In addition, animal studies have provided little insight into the mechanisms of toxicity because no animal model exhibits the symptoms of TOS. Researchers of Eosinophilia- Myalgia Syndrome (EMS) are in a similar quandary. EMS occurred in the United States in 1989, and the symptoms of this illness parallel TOS quite closely. The MRL/lpr mouse model has been suggested as a possible model for immunotoxicity caused by environmental exposure. Since the symptoms of most chronic phase patients appeared to be immunologically mediated, the MRL/lpr mouse was chosen as the animal model for the present experiment. Sixty two mice were used. Groups often mice were gavaged with three different dose levels of 2% aniline denatured low-erucic acid rapeseed oil (Canola oil) and mercuric chloride as a positive control. Ten mice were untreated as a naive control. Two mice were sacrificed upon arrival as a negative control. All mice treated with toxic oil displayed a decreased rate of weight gain relative to the naive control. Serum antinuclear antibodies (ANA) were detected using indirect immunofluorescence, and anti-type IV collagen antibodies (ACA) were detected using an ELISA technique. The mice receiving toxic oil displayed increased serum ANA titers relative to the naive control. However, there did not appear to be a relationship between toxic oil dose and ANA titer. All animals receiving oil displayed decreased serum ACA titers relative to the naive control. In this case, a direct relationship existed between ACA [p.2 of abstract missing].
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