Assessment of the quantitative fluorescent antibody technique and chemotherapy for the detection and control of Renibacterium salmoninarum in salmonid fishes Public Deposited

http://ir.library.oregonstate.edu/concern/graduate_thesis_or_dissertations/44558h52v

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  • Detection and treatment of bacterial kidney disease (BKD) was investigated. Experiments were conducted to evaluate the quantitative, fluorescent antibody technique (QFAT) that is used to detect, identify, and quantify both typical and 'bar form' Renibacterium salmoninarum cells. Smears of kidney tissue from naturally and artificially infected salmonids, both with and without chemotherapy, were quantitatively examined throughout the course of R. salmoninarum infections. Detection and quantification by QFAT has been reported to provide assessments of prevalence and severity of R. salmoninarum of individual fish. These assessments and the occurrence of 'bar forms' of R. salmoninarum have been used as an indication of recovery within a population. 'Bar forms' were observed in kidney tissue smears of fish that survived bacterial challenge when treated with erythromycin. The 'bar form' was also detected when rainbow trout were artificially infected with lower doses of live R . salmoninarum and in fish that were injected with irradiation-inactivated R. salmoninarum cells. By examining R. salmoninarum cultures in vitro by QFAT, it was determined that 'bar forms' did not occur on artificial media even when antibiotics were incorporated into the agar. When QFAT was compared to direct fluorescent antibody technique (DFAT) and quantitative enzyme linked immunosorbent assay (ELISA), it was determined that QFAT had similar sensitivity as ELISA but was more sensitive than DFAT. QFAT was also used to predict minimum mortality. Experiments were also conducted to evaluate drug regimes to treat both artificial and natural R. salmoninarum infections. Erythromycin was administered by intraperitoneal injection in different doses and at selected days post infection. Erythromycin decreased percent mortality and increased mean day to death, but did not completely eradicate R. salmoninarum from infected test animals. Sarafloxacin and erythromycin were incorporated into daily ration of artificially infected test animals. Contrary to erythromycin, sarafloxacin did not decrease mortality or increase mean day to death when tested in vivo against R. salmoninarum. A new drug, A-77143, was tested in vitro to determine if it was bactericidal and its minimum inhibitory concentration. When A- 77143 was compared to other antibiotics, it had a relatively low minimum inhibitory concentration and was shown to be bactericidal against the eight strains of R. salmoninarum tested.
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