Graduate Thesis Or Dissertation

 

Evaluation of media inhibitory for lactic streptococcal bacteriophages Public Deposited

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  • Seven commercially prepared brands of lactic streptococcal phage inhibitory media were obtained from their manufacturers. Comparisons between the different media were made with respect to buffering capacity, ability to support lactic streptococcal growth, ability to suppress bacteriophage replication, and ability to support Leuconostoc growth. Nonfat milk was used during experiments as a control. Results indicated that only 2 of the 7 phage inhibitory media tested were adequate in preventing phage proliferation, and that the capacity of any one medium to stop phage growth was directly related to its buffering capacity. Compositional data indicated that those media which were most effective in preventing phage replication contained better nutrients to support streptococcal growth, thus overcoming the effects of high phosphate and/or citrate concentrations which were found to depress growth. The medium which supported the best streptococcal growth as compared to nonfat milk was intermediate in suppressing phage proliferation. The four most effective media each contained citrate buffers, whereas the three least effective media lacked this compound. The most effective medium also contained hydrolyzed cereal solids to enhance streptococcal growth. Leuconostoc growth was inversely related to the phage inhibitory properties of the different media, perhaps due to phosphates and/or citrates sequestering manganese, which is essential for Leuconostoc growth. The use of β - glycerophosphate was also tested as a milk buffering agent and a supplement to phage inhibitory media. Lactic streptococci, when propagated in nonfat milk supplemented with β - glycerophosphate (0. 088M), were more active upon transfer to milk than control cultures. β - glycerophosphate, when added to phage inhibitory media (0. 044M), also increased the effectiveness of 6 of the 7 media with respect to phage replication. For example, this effect was maximum for one medium where the buffer enhanced the inhibitory ability by almost 99 %; the minimum positive effect with another brand was 27%.
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