Graduate Thesis Or Dissertation

 

Interfacing a fluorescence anisotropy spectrophotometer to a PDP-8/E computer Public Deposited

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https://ir.library.oregonstate.edu/concern/graduate_thesis_or_dissertations/4b29b835r

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  • A fluorescence anisotropy spectrophotometer known by the acronym GUS has been in use at Oregon State University in the department of biochemistry and biophysics for several years under the supervision of Dr. Irvin Isenberg. Dr. Isenberg wished to improve the accuracy, versatility, speed of data acquisition, and ease of operation by using a PDP-8/E computer to control the apparatus, collect data, and compute the desired results. Formerly the apparatus was controlled manually and data conditioning was done primarily by analog means. The author was given the task of designing the interface between GUS and the PDP-8/E computer given the following conditions: I. Two analog signals from GUS must be conditioned and connected to the ADO1 analog to digital converter supplied with the PDP-8/E computer. II. A reference signal is available from the mechanical light chopper of GUS, and data sampling must be synchronized with this reference. III. A simulator must be built to provide controllable test signals with noise for purposes of testing the computer operation under known input conditions. IV. An automatic control must be built for initiating the computer program periodically at a preselected time interval for the purpose of observing time varying samples. V. In addition the ADO1 analog to digital converter needs substantial modification to make it compatible with the rest of the system. This paper describes the implementation of the system as outlined above.
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