Development and application of SSR markers for measuring gene flow in Douglas-fir Public Deposited

http://ir.library.oregonstate.edu/concern/graduate_thesis_or_dissertations/4b29b8468

Descriptions

Attribute NameValues
Creator
Abstract or Summary
  • Gene flow is a major evolutionary force and an important factor in the breeding and conservation of forest trees. I studied the applicability of SSR markers for measuring pollen-mediated gene flow (i.e., pollen flow) in Douglas-fir (Pseudotsuga menziesii [Mirb.] Franco). I developed SSR markers, tested alternative approaches for measuring pollen flow using SSR markers, then measured pollen contamination and characterized within-block mating patterns in one block of a seed orchard complex. Useful markers were developed from 4.1% of the SSR sequences screened. The 22 markers obtained are among the most informative genetic markers available for Douglas-fir. The observed heterozygosity and the number of alleles per marker averaged 0.855 (SE=0.020) and 23 (SE=1.6), respectively. Mistyping (i.e., false identification of genotypes) results in overestimating pollen flow. Requiring multiple mismatches for paternity exclusion, while assuring that the probability of detecting immigrant genotypes is high, results in accurate estimates of pollen flow. I developed and made available the Pollen Flow (PFL) computer program, which performs paternity exclusion and measures pollen flow based on multiple fatheroffspring mismatches. Pollen contamination was consistently high in all three years in which seed crops were sampled from the orchard block (mean = 35.3%). Levels of pollen contamination varied substantially among clones, and were significantly higher in clones with early female receptivity (mean = 55.5%) than in those with intermediate (mean = 36.4%) or late (mean = 28.3%) female receptivity. Seeds resulting from self-fertilization were rare (mean = 1.8%). Differences in the relative paternal contributions of the clones in the block were greater than ten-fold, and there was preferential mating among parents with similar floral phenology. Information from analyses of SSR data can be used to minimize pollen contamination and improve within-orchard mating patterns. Furthermore, SSRs can be used to advance knowledge of gene flow in natural populations. The availability of large sets of highly variable SSRs makes it possible to perform landscape-scale studies of gene flow and better understand the interactions between gene flow and adaptation. These studies will ultimately provide a basis for decisions in breeding and conservation programs.
Resource Type
Date Available
Date Copyright
Date Issued
Degree Level
Degree Name
Degree Field
Degree Grantor
Commencement Year
Advisor
Academic Affiliation
Non-Academic Affiliation
Subject
Rights Statement
Language
Digitization Specifications
  • Master files scanned at 600 ppi (256 Grayscale, 24-bit Color) using Capture Perfect 3.0 on a Canon DR-9080C in TIF format. PDF derivative scanned at 300 ppi (256 B&W, 24-bit Color), using Capture Perfect 3.0, on a Canon DR-9080C. CVista PdfCompressor 4.0 was used for pdf compression and textual OCR.
Replaces
Additional Information
  • description.provenance : Made available in DSpace on 2009-10-22T17:52:50Z (GMT). No. of bitstreams: 1 SlavovGanchTrifonov2004.pdf: 918553 bytes, checksum: 77965428d1dfb6c861c3d0eb01d76eb1 (MD5)
  • description.provenance : Approved for entry into archive by Patricia Black(patricia.black@oregonstate.edu) on 2009-10-22T17:49:52Z (GMT) No. of bitstreams: 1 SlavovGanchTrifonov2004.pdf: 918553 bytes, checksum: 77965428d1dfb6c861c3d0eb01d76eb1 (MD5)
  • description.provenance : Submitted by Eric Hepler (ehscanner@gmail.com) on 2009-10-22T16:33:44Z No. of bitstreams: 1 SlavovGanchTrifonov2004.pdf: 918553 bytes, checksum: 77965428d1dfb6c861c3d0eb01d76eb1 (MD5)
  • description.provenance : Approved for entry into archive by Patricia Black(patricia.black@oregonstate.edu) on 2009-10-22T17:52:50Z (GMT) No. of bitstreams: 1 SlavovGanchTrifonov2004.pdf: 918553 bytes, checksum: 77965428d1dfb6c861c3d0eb01d76eb1 (MD5)
  • description.provenance : Rejected by Patricia Black(patricia.black@oregonstate.edu), reason: Insert p.57 on 2009-10-21T16:50:16Z (GMT)
  • description.provenance : Submitted by Eric Hepler (ehscanner@gmail.com) on 2009-10-20T22:09:06Z No. of bitstreams: 1 SlavovGanchTrifonov2004.pdf: 876921 bytes, checksum: f3e0c926f02a2d20654851a9f1297bd1 (MD5)

Relationships

Parents:

This work has no parents.

Last modified

Downloadable Content

Download PDF

Items