The effect of erythorbic acid on the determination of ascorbic acid levels in selected foods by high performance liquid chromatography and spectrophotometry Public Deposited

http://ir.library.oregonstate.edu/concern/graduate_thesis_or_dissertations/4f16c5724

Descriptions

Attribute NameValues
Creator
Abstract or Summary
  • A high performance liquid chromatography (HPLC) procedure using a LiChrosorb-NH₂ column and a eluant buffer of 75:25 (v/v) of acetonitrile:0.05 M KH₂P0₄, pH 5.95, was developed for the successful separation and determination of ascorbic and erythorbic acids in selected food samples. Application of the method, which is sensitive, rapid and simple, for the analysis of ascorbic and erythorbic acids in frozen apples, potato products, fruit and vegetable concentrated juices, frozen juices, natural and artificial flavor drink mixes, and Hi-C drinks gave satisfactory results. Dehydroascorbic and dehydroerythorbic acids in these samples could also be determined after reduction with dithiothreitol. It was verified by HPLC that the presence of erythorbic acid affected the determination of ascorbic acid levels by the spectrophotometric method by causing elevated absorbance readings and hence, abnormally high values. Erythorbic acid seriously affects the true determination of ascorbic acid contents in food samples by the spectrophotometric method. The use of HPLC is recommended for the routine analysis of ascorbic acid of those samples containing both ascorbic and erythorbic acids.
Resource Type
Date Available
Date Copyright
Date Issued
Degree Level
Degree Name
Degree Field
Degree Grantor
Commencement Year
Advisor
Committee Member
Academic Affiliation
Non-Academic Affiliation
Subject
Rights Statement
Peer Reviewed
Language
Digitization Specifications
  • File scanned at 300 ppi (Monochrome) using Scamax Scan+ V.1.0.32.10766 on a Scanmax 412CD by InoTec in PDF format. LuraDocument PDF Compressor V.5.8.71.50 used for pdf compression and textual OCR.
Replaces
Additional Information
  • description.provenance : Made available in DSpace on 2012-01-26T19:44:55Z (GMT). No. of bitstreams: 1 TUANSHENHSIU1986.pdf: 817944 bytes, checksum: 5da26858bac19b331b836109374bfec3 (MD5) Previous issue date: 1986-03-18
  • description.provenance : Submitted by Erin Clark (ecscannerosu@gmail.com) on 2011-12-27T16:31:53Z No. of bitstreams: 1 TUANSHENHSIU1986.pdf: 817944 bytes, checksum: 5da26858bac19b331b836109374bfec3 (MD5)
  • description.provenance : Approved for entry into archive by Patricia Black(patricia.black@oregonstate.edu) on 2012-01-03T16:47:42Z (GMT) No. of bitstreams: 1 TUANSHENHSIU1986.pdf: 817944 bytes, checksum: 5da26858bac19b331b836109374bfec3 (MD5)
  • description.provenance : Approved for entry into archive by Patricia Black(patricia.black@oregonstate.edu) on 2012-01-26T19:44:55Z (GMT) No. of bitstreams: 1 TUANSHENHSIU1986.pdf: 817944 bytes, checksum: 5da26858bac19b331b836109374bfec3 (MD5)

Relationships

In Administrative Set:
Last modified: 08/08/2017

Downloadable Content

Download PDF
Citations:

EndNote | Zotero | Mendeley

Items