Graduate Thesis Or Dissertation

 

Genetics of sexuality and population genetics of Phellinus weirii Público Deposited

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https://ir.library.oregonstate.edu/concern/graduate_thesis_or_dissertations/4j03d218k

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  • Phellinus weirii (Murr.) Gilbertson, cause of laminated root and butt rot, is a heterothallic basidiomycete lacking clamp connections. Two biological species groups of the fungus have been hypothesized, the Douglas-fir and cedar-types, on the basis of physiological, morphological and epidemiological differences. Single-spore and vegetative isolates differ in cultural morphology, nuclear composition and growth rates, but variation among isolates makes it impossible to accurately identify the ploidy of individuals. Pairings of single-spore isolates in culture produce a wide range of reactions, from formation of darkly pigmented barrage lines to no visible change in morphology. Back-pairings of interacted mycelium from single-spore pairings with completely compatible homokaryons reveal that P. weirii follows a bipolar, multiallelic system of mating compatibility. Single-spore and homokaryon-heterokaryon pairings followed by back-pairings demonstrate the genetic isolation between Douglas-fir and cedar-type isolates, confirming the biological species hypothesis. Existence of a third Asian species is suggested by the lack of interaction of two Japanese isolates with both Douglas-fir and cedar-type tester homokaryons. Protein banding patterns obtained by SDS polyacrylimide gel electrophoresis show overall similarity between the three types but differ at several band locations. The Douglas-fir-type isolates were found on Douglas-fir and six other host species, including western redcedar. The cedar-type isolates were mostly confined to western redcedar, but were also identified on western hemlock and grand fir. The geographic ranges of the Douglas-fir and cedar-type Phellinus weirii overlap in much of the Douglas-fir region, indicating that the emerging biological species groups are likely the result of host specialization rather than geographic separation.
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