Graduate Thesis Or Dissertation

 

The effect of potassium pentachlorophenate on selected enzymes in fish Public Deposited

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  • Research on chemicals used as pesticides has been intensified because of current interest in the possible long range effects on the biota. It is vitally important to understand the action of these chemicals on the metabolism of animals and plants which have direct or indirect contact with pesticides. It has been shown that pentachlorophenol (PCP) is a potent uncoupling agent of oxidative phosphorylation. The effect of PCP on four selected enzymes in the muscles of cichlids and goldfish was studied. Aldolase, glutamic oxalacetic transaminase (GOT), glutamic pyruvic transaminase (GPT) and lactic dehydrogenase (LD) were apparently inhibited in vitro by potassium pentachlorophenate (KPCP). The inhibition increased with the increasing of the concentration of KPCP. Isocitric dehydrogenase (ICD) in contrast showed a gradual increase in activity with KPCP up to 150 ppm. For in vivo studies, acetone powder extracts of cichlids exposed to 0, 0, 0.1 and 0.2 ppm KPCP for 0, 1, 2 and 4 days and acetone powder extracts of goldfish having been exposed to 0.0, 0.1, 0.2 and 0.4 ppm KPCP for 0 and 4 days were used. The results showed that there was a dual effect of KPCP, giving stimulation or inhibition of aldolase, GOT and GPT in cichlids muscle, and aldolase, GOT and LD in goldfish muscle depending on the concentration. The increasing inhibition of these enzymes in vivo is directly proportional to the concentration of KPCP. This might be an indication that the inhibition was non-competitive. The lethal dosage of KPCP for goldfish has been found as 0.46 ppm or 1.43 x 10⁻⁶ M KPCP.
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