Isozyme banding patterns and embryo development in interspecific crosses of Phaseolus Public Deposited

http://ir.library.oregonstate.edu/concern/graduate_thesis_or_dissertations/4t64gr30n

Descriptions

Attribute NameValues
Creator
Abstract or Summary
  • Interspecific hybridization between Phaseolus vulgaris and P. coccineus results in the formation of either mature F₁ seeds or abnormal embryos, depending on the direction of the cross. However, normal as well as abnormal embryos occur in the reciprocal F₂ populations. The exact cause of abnormal embryo development is unknown. To determine if there is a correlation between the genomic composition and embryo development (normal vs. abnormal), isozyme banding patterns were examined in the reciprocal F₁ and F₂ progenies of P. vulgaris cv. Great Northern (GN) and P. coccineus cv. Scarlet Runner (SR). Three enzyme systems, acid phosphatase (AP), esterase (EST), and malate dehydrogenase (MDH) were analysed in extracts of immature embryos at the cotyledonary stage. Parental species and reciprocal F₁ and F₂ embryos exhibited clear differences in electrophoretic patterns. The embryos of reciprocal crosses displayed banding patterns characteristic for their developmental type (normal or abnormal). AP and MDH zymograms of normal embryos were closer to those of the GN parent, whereas abnormal embryos had isozyme bands similar to those of SR. Differences in EST banding patterns were apparent between normal and abnormal F₂ embryos, however, these patterns did not resemble either parent. The results indicate that embryo development can be correlated with particular isozyme patterns which may at least in part reflect the genetic combination. They also seem to substantiate the hypothesis that the skewed distributions of quantitative traits in interspecific progeny populations could be related to selective survival of seeds containing normal embryos.
Resource Type
Date Available
Date Copyright
Date Issued
Degree Level
Degree Name
Degree Field
Degree Grantor
Commencement Year
Advisor
Committee Member
Academic Affiliation
Non-Academic Affiliation
Subject
Rights Statement
Peer Reviewed
Language
Digitization Specifications
  • File scanned at 300 ppi (Monochrome, 256 Grayscale) using Scamax Scan+ V.1.0.32.10766 on a Scanmax 412CD by InoTec in PDF format. LuraDocument PDF Compressor V.5.8.71.50 used for pdf compression and textual OCR.
Replaces
Additional Information
  • description.provenance : Submitted by Erin Clark (ecscannerosu@gmail.com) on 2011-11-16T17:56:43Z No. of bitstreams: 1 GUOMEI1989.pdf: 642131 bytes, checksum: b50f6628d991a1428a3d562562a15d6f (MD5)
  • description.provenance : Approved for entry into archive by Patricia Black(patricia.black@oregonstate.edu) on 2011-11-17T22:29:56Z (GMT) No. of bitstreams: 1 GUOMEI1989.pdf: 642131 bytes, checksum: b50f6628d991a1428a3d562562a15d6f (MD5)
  • description.provenance : Made available in DSpace on 2011-11-17T22:29:56Z (GMT). No. of bitstreams: 1 GUOMEI1989.pdf: 642131 bytes, checksum: b50f6628d991a1428a3d562562a15d6f (MD5) Previous issue date: 1989-03-14
  • description.provenance : Approved for entry into archive by Patricia Black(patricia.black@oregonstate.edu) on 2011-11-16T21:43:29Z (GMT) No. of bitstreams: 1 GUOMEI1989.pdf: 642131 bytes, checksum: b50f6628d991a1428a3d562562a15d6f (MD5)

Relationships

In Administrative Set:
Last modified: 08/15/2017

Downloadable Content

Download PDF
Citations:

EndNote | Zotero | Mendeley

Items