Graduate Thesis Or Dissertation
 

The effect of ascorbate on enzymatic nitroglycerin bioactivation as measured by direct LC-MS/MS determination of nitrite

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  • This work sought to characterize a link between Vitamin C and nitroglycerin at the level of enzymatic bioactivation. Before we could proceed, we needed to develop a method that sensitively and selectively identified the product of bioactivation, nitrite. We successfully adapted traditional diaminonaphthalene derivatization with the use of stable isotope liquid chromatography tandem mass spectrometry that was free from interferences commonly encountered, including ascorbate and high nitrite background. We then applied our method to enzyme incubations with aldehyde dehydrogenase (ALDH) before moving on to incubations with xanthine oxidase. We successfully determined a link between Vitamin C and GTN bioactivation only in incubations with xanthine oxidase, but saw no evidence of ALDH catalyzed nitrite production.
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Déclaration de droits
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file details HardardtElizabethA2014.pdf 2017-08-22 Public Télécharger