Graduate Thesis Or Dissertation
 

Formulation studies of lactic acid-producing bacteria for oral administration

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https://ir.library.oregonstate.edu/concern/graduate_thesis_or_dissertations/5138jj826

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  • Effects of lactobacilli in enteric coated capsules were studied in piglets (Chapter I). Lactobacillus acidophilus and Lactobacillus bulgaricus isolated from a commercial product were formulated in capsules, enteric coated, and then given to both Escherichia coli-challenged and unchallenged baby pigs. Decreased incidence and severity of diarrhea, and decreased weight deceleration showed that the enteric coated lactobacilli had more beneficial effects than nonenteric coated bacteria. A methodology for microencapsulating live lactobacilli with aqueous enteric polymers has been developed successfully (Chapter II). Short processing times and incorporation of talc in the coating chamber were found to be critical to maintain viability. Talc smoothed the coating, made application of coating solution easier, and stabilized previously adsorbed microorganisms. Dissolution tests and scanning electron microscopy were used to evaluate the homogeneity of the aqueous enteric polymer film. The enteric coated microencapsulated dosage form may not only provide more uniform action, but also protect lactobacilli from oxygen and increase their shelf-life. Optimization of the growth of bifidobacteria, a genus closely related to lactobacilli, in modified milk was studied (Chapter III). A buffer system and a chemical reaction were provided simultaneously to neutralize acids produced and expell oxygen from the medium. A lab-scale fermenter was designed to quantify this anaerobic condition. Enteric coated capsules containing bifidobacteria were prepared and evaluated for microorganism survival in gastric fluid followed by intestinal fluid.
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