Graduate Thesis Or Dissertation

 

Hydrolytic methods for the quantification of fructose-equivalents in herbaceous biomass Public Deposited

Analytics

Downloadable Content

Download PDF
https://ir.library.oregonstate.edu/concern/graduate_thesis_or_dissertations/5d86p343h

Descriptions

Attribute NameValues
Creator
Abstract
  • A low, but significant, fraction of the carbohydrate portion of herbaceous biomass may be composed of fructose/fructosyl-containing components (“fructose equivalents”); such carbohydrates include sucrose, fructo-oligosaccharides, and fructans. Standard methods used for the quantification of structural-carbohydrate-derived neutral monosaccharide-equivalents in biomass are not particularly well suited for the quantification of fructose equivalents due to the inherent instability of fructose in conditions commonly used for hemicellulose/cellulose hydrolysis (> 80% degradation of fructose standards treated at 4% sulfuric acid, 121oC, 1 hr). Alternative time, temperature and acid concentration combinations for fructan hydrolysis were considered using model fructans (inulin, β-2,1 and levan, β-2,6) and a grass seed straw (Tall Fescue, Festuca arundinacea) as representative feedstocks. The instability of fructose, relative to glucose and xylose, at higher acid/temperature combinations is demonstrated, all rates of fructose degradation being acid and temperature dependent. Fructans are shown to be completely hydrolyzed at acid concentrations well below that used for the structural carbohydrates, as low as 0.2%, at 121oC for 1 hr. Lower temperatures are also shown to be effective, with corresponding adjustments in acid concentration and time. Thus, fructans can be effectively hydrolyzed under conditions where fructose degradation is maintained below 10%. Hydrolysis of the β-2,1 fructans at temperatures ≥ 50oC, at all conditions consistent with complete hydrolysis, appear to generate difructose dianhydrides. These same compounds were not detected upon hydrolysis of levan, sucrose, or straw components. It is suggested that fructan hydrolysis conditions be chosen such that hydrolysis goes to completion, fructose degradation is minimized, and difructose dianhydride production is accounted for.
License
Resource Type
Date Available
Date Issued
Degree Level
Degree Name
Degree Field
Degree Grantor
Commencement Year
Advisor
Committee Member
Academic Affiliation
Non-Academic Affiliation
Keyword
Subject
Rights Statement
Publisher
Language
Replaces
Additional Information
  • description.provenance : Rejected by Julie Kurtz(julie.kurtz@oregonstate.edu), reason: Rejecting to convert to one PDF. If you need assistance without affecting the page numbering you can email your Word documents to - Teresa.Preddy@oregonstate.edu. She will email you back one PDF. Once revised, open the item that was rejected, replace the attached file with the revised file, and resubmit. Thanks, Julie on 2008-07-10T00:55:27Z (GMT)
  • description.provenance : Submitted by Stefanie Nguyen (nguyens@onid.orst.edu) on 2008-07-18T19:33:40Z No. of bitstreams: 1 nguyens_masterthesis.pdf: 397731 bytes, checksum: 928d4e2b3d14ec701a5342d038e3a4c8 (MD5)
  • description.provenance : Approved for entry into archive by Julie Kurtz(julie.kurtz@oregonstate.edu) on 2008-07-18T20:47:35Z (GMT) No. of bitstreams: 1 nguyens_masterthesis.pdf: 397731 bytes, checksum: 928d4e2b3d14ec701a5342d038e3a4c8 (MD5)
  • description.provenance : Approved for entry into archive by Linda Kathman(linda.kathman@oregonstate.edu) on 2008-07-22T13:58:36Z (GMT) No. of bitstreams: 1 nguyens_masterthesis.pdf: 397731 bytes, checksum: 928d4e2b3d14ec701a5342d038e3a4c8 (MD5)
  • description.provenance : Submitted by Stefanie Nguyen (nguyens@onid.orst.edu) on 2008-07-03T21:31:30Z No. of bitstreams: 2 pretext.pdf: 70182 bytes, checksum: ec284c8625557c2973a2beaab9190111 (MD5) thesis0701_final.pdf: 516139 bytes, checksum: 46b8d6e47d81ca546e9bbde5576bd274 (MD5)
  • description.provenance : Made available in DSpace on 2008-07-22T13:58:37Z (GMT). No. of bitstreams: 1 nguyens_masterthesis.pdf: 397731 bytes, checksum: 928d4e2b3d14ec701a5342d038e3a4c8 (MD5)

Relationships

Parents:

This work has no parents.

In Collection:

Items

Thumbnail Title Date Uploaded Visibility Actions
nguyens_masterthesis.pdf 2017-08-19 Public