Graduate Thesis Or Dissertation
 

Improved detection methods and physiological studies of staphylococcal enterotoxin

公开 Deposited

可下载的内容

下载PDF文件
https://ir.library.oregonstate.edu/concern/graduate_thesis_or_dissertations/5h73pz94v

Descriptions

Attribute NameValues
Creator
Abstract
  • The purpose of this study was to develop means to facilitate the detection of staphylococcal enterotoxin and to elucidate factors that influence enterotoxin formation. It was found that semipurification flat-gel electrophoresis in a water-cooled apparatus was more efficient than CM-cellulose and Sephadex column chromatography currently used to separate toxin from soluble food constituents. The electrophoretic process was less time consuming, less expensive and adaptable to mulitple sample analyses. A technique combining isoelectric focusing in a polyacrylamidestabilized ampholyte solution followed by electro-osmophoresis was 5ucce ssful in reducing the time required for the separation of enteroioxii. l. A and the formation of a specific immuno-precipitate. It was possible by this method to assay enterotoxin A at a concentration of 0.5 μg /ml in five hours. Inhibitor studies were conducted on enterotoxin A and B production by Staphylococcus aureus 265-1 and 14458, respectively. The elaboration of enterotoxin A and B was inhibited by streptomycin sulfate, NaF, chioraniphenicol, KC1 and bacitracin. Inhibition by KC1 and NaF was reversed by Mg⁺⁺ . Penicillin G inhibited toxin production by S. aureus 14458 but not cell growth, whereas both toxin elaboration and growth were inhibited in studies with S. aureus 265-1, Enterotoxin B produced by S. aureus 1445 appeared during the latter part of the exponential growth phase. Nonreplicating cells at a concentration of 8 x 10¹⁰/ml were found capable of producing toxin in the presence of glucose and water alone. Chloramphenicol did not inhibit toxin formation in nonreplicating cells. Data suggested the existence of a toxin precursor pool and that toxin excretion may be an adjustment to static growth conditions.
Resource Type
Date Available
Date Issued
Degree Level
Degree Name
Degree Field
Degree Grantor
Commencement Year
Advisor
Academic Affiliation
Non-Academic Affiliation
Subject
权利声明
Publisher
Peer Reviewed
Language
Digitization Specifications
  • File scanned at 300 ppi (Monochrome, 8-bit Grayscale, 24-bit Color) using ScandAll PRO 1.8.1 on a Fi-6670 in PDF format. CVista PdfCompressor 4.0 was used for pdf compression and textual OCR.
Replaces

关联

Parents:

This work has no parents.

属于 Collection:

单件

缩略图 标题 上传日期 公开度 行动
file details KimbleCharlesE1970.pdf 2017-08-14 公开 下载