Graduate Thesis Or Dissertation

 

Transcript analysis of Feldmannia Sp. virus, FsV : characterization of the major capsid protein gene and its relationship to known viruses Öffentlichkeit Deposited

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https://ir.library.oregonstate.edu/concern/graduate_thesis_or_dissertations/5m60qv47q

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  • The Feldmannia sp. virus is a large icosahedral virus that persistently infects marine brown alga Feldmannia sp.. So far, there is no information available about viral genome replication, gene structure and gene expression in this unique viral-host system. The purpose of this study was to characterize the general features of viral transcripts in the virus producing sporophyte plants. Northern analysis, using four cosmid clones that cover the entire viral genome, showed that there were six major transcripts and at least eighteen minor transcripts in the virus producing sporophyte plants. These transcripts are not evenly distributed in the viral genome. A 5.7 kb BamHI fragment-R was found to encode a 1.5 kb and a 0.9 kb major transcript, and those two major transcripts were chosen for detailed sequence analysis. The 1.5 kb transcript was identified as the putative major capsid protein (MCP) gene. The FsV MCP has significant similarity with the major capsid protein of Chlorella virus-PBCV-1 and with iridoviruses, fish lymphocystis disease virus, frog virus 3, and with African swine fever virus.
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