Interactions between paired cultures as a basis for differentiating compatibility genotypes of Fomes cajanderi Karst. from glaze-damaged Douglas-fir Public Deposited

http://ir.library.oregonstate.edu/concern/graduate_thesis_or_dissertations/6108vd61w

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  • A study was undertaken to determine the reliability of the formation of a line of demarcation between paired cultures of Fomes cajanderi Karst. as an indicator of dissimilar compatibility genotypes. The results were applied to an assay of the number and location of compatibility genotypes in each of four glaze-damaged, infected Douglas-firs. Spores shed from sporophores formed in culture enabled development of pedigreed mono- and dikaryotic lines. Di-mon, and dikaryon pairings of known relationship were made in culture and data recorded as to the frequency of formation of the line of demarcation. The results indicate the line of demarcation in paired culture to be a highly reliable diagnostic feature of the presence of dissimilar compatibility genotypes. Variations in the intensity of expression of the line of demarcation occur when sibrelated dikaryons are paired, but when mycelia not closely related are paired there is little deviation from the distinct, dark line common to pairings of diverse relationship. Four glaze-damaged Douglas-firs infected by F. cajanderi 22 years before initiation of the study, were examined to determine the number of compatibility genotypes present in the decay column of each tree. Sections of the bole were removed at intervals from along the decay column of each tree, placed in plastic sacks and incubated for five months. Isolations from mycelia growing on the surfaces of these sections were paired in culture to determine the relationships involved. On the basis of the line of demarcation in paired culture, the trees contained from one to eight compatibility genotypes, representing at least as many successful infections in each tree. Variation in cultural appearance was found between ramets of the same compatibility genotype. Observations indicated the presence of a zone of wood in the inner sapwood of the trees examined that is resistant or impervious to decay by organisms from either the heartwood or outer sapwood. However, "mycelial bridges ", developed by F. cajanderi crossed this zone.
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