Graduate Thesis Or Dissertation
 

Extraction of proteins from wood samples colonized by bioprotectants and/or stain fungi

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  • Biological stain (blue stain) reduces wood value and prevents its use in many structural applications. Increasing environmental concerns have restricted the chemicals available for controlling this damage. As a result, biological control has received more interest. The application of these systems to wood has been limited due to their inability to reliably produce effective stain control under mill conditions. Many of these problems stem from a poor understanding of how these organisms function as bioprotectants. In this thesis, a technique was identified to extract proteins from ponderosa pine sapwood samples colonized by bioprotectants and/or sapstain fungi. These extracts could be used to study the effects of the bioprotectants on various target stain fungi. The effects of extraction media and sample incubation period on protein recovery and enzyme activity in extracts were evaluated. Protein recovery and activity in extracts were evaluated. Protein recovery and enzyme activity were affected by the extraction time, the medium conditions of the bioprotectants and the sample incubation conditions. The greatest protein recovery occurred, under the conditions of this study, when wood samples were incubated for thirty days and extracted for twelve hours using 50 mM sodium acetate buffer at pH 5.0 with 0.05 % Tween 80. The surfactant, Tween 80, greatly enhanced protein recovery. Longer incubation time (thirty days) was associated with greater produce cellobiase and xylosidase activities but, in contrast, glucosidase activities were greater with shorter incubation time (ten days), under the conditions of this study.
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