Graduate Thesis Or Dissertation

 

Purification and properites of the polygalacturonases produced by Fusarium oxysporum f. sp. lycopersici Public Deposited

Downloadable Content

Download PDF
https://ir.library.oregonstate.edu/concern/graduate_thesis_or_dissertations/6682x679s

Descriptions

Attribute NameValues
Creator
Abstract
  • This study was undertaken to determine the sorts of polygalacturonases present in the culture filtrate of Fusarium oxysporum f. sp. lycopersici, to purify and characterize these enzymes, and to study any complexes that may form between polygalacturonase and carbohydrate not degraded by the enzyme. The polygalacturonase in culture filtrate consists of at least two types of enzymes separable on DEAE cellulose. The major fraction is not adsorbed to DEAE cellulose, while the minor fraction must be eluted with 0.4 M NaCl. Both may be separated from other pectolytic enzymes by column chromatography on CM cellulose and hydroxylapatite. The major enzyme fraction contains a single endopolygalacturonase which behaves as a homogenous protein during chromatography on Sephadex G-75 and Bio-Rex 70 and in polyacrylamide electrophoresis. The pH optimum of this enzyme is 4.0, and its activity is inhibited by mono-galacturonic acid but stimulated by a mixture of mono- through tetra-galacturonic acids. The minor fraction contains a similar polygalacturonase but with some different properties as determined by chromatography on Sephadex G-75 and specific activity. However, other evidence suggests that the apparent negative charge of this polygalacturonase, which was the original basis for separation of the minor fraction from the major fraction, changes during purification and may arise from uronides associated with the polygalacturonase.
Resource Type
Date Available
Date Copyright
Date Issued
Degree Level
Degree Name
Degree Field
Degree Grantor
Commencement Year
Advisor
Academic Affiliation
Non-Academic Affiliation
Subject
Rights Statement
Peer Reviewed
Language
Digitization Specifications
  • File scanned at 300 ppi (Monochrome) using ScandAll PRO 1.8.1 on a Fi-6770A in PDF format. CVista PdfCompressor 5.0 was used for pdf compression and textual OCR.
Replaces
Additional Information
  • description.provenance : Made available in DSpace on 2014-02-10T16:09:24Z (GMT). No. of bitstreams: 1 HarmanGaryE1970.pdf: 508475 bytes, checksum: 8e289a3e7a32089d1355eaaa60c8c4cd (MD5) Previous issue date: 1969-10-08
  • description.provenance : Approved for entry into archive by Katy Davis(kdscannerosu@gmail.com) on 2014-02-10T16:09:24Z (GMT) No. of bitstreams: 1 HarmanGaryE1970.pdf: 508475 bytes, checksum: 8e289a3e7a32089d1355eaaa60c8c4cd (MD5)
  • description.provenance : Approved for entry into archive by Patricia Black(patricia.black@oregonstate.edu) on 2014-02-03T19:20:31Z (GMT) No. of bitstreams: 1 HarmanGaryE1970.pdf: 508475 bytes, checksum: 8e289a3e7a32089d1355eaaa60c8c4cd (MD5)
  • description.provenance : Submitted by Madison Medley (mmscannerosu@gmail.com) on 2014-02-03T19:01:25Z No. of bitstreams: 1 HarmanGaryE1970.pdf: 508475 bytes, checksum: 8e289a3e7a32089d1355eaaa60c8c4cd (MD5)

Relationships

Parents:

This work has no parents.

Items