Graduate Thesis Or Dissertation
 

Toxicity of methyl bromide to fungi inhabiting Dahurian larch wood

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  • Concerns over the possibility of exotic pest introductions from eastern Russia to the West Coast of the United States due to proposed log imports raises the question of the effectiveness of possible mitigation measures. Toxicity of methyl bromide to representative pathogenic fungi was tested by exposing Armillaria ostoyae, Heterobasidion annosum, Lachnellula wilkommii and Leptographium wageneri grown in one centimeter media-amended Dahurian larch (Larix gmelinii) wood cubes to initial concentrations of 0, 79 or 237 mg/l of methyl bromide for 8, 24 or 72 hours. All fumigations were performed at 18° C in sealed glass jars. Leakage of fumigant from the jars and sorption into the cubes was closely monitored. After fumigation, cubes were quartered and plated on malt agar. The percentage of cube quarter sections with no visible growth after two weeks was used as a bioassay of fumigation efficacy. Survival was noted at concentration time values (CxT) as high as 1267 mgh/l for Armiliaria ostoyae, 3006 mgh/l for Heterobasidion annosum, 1230 mgh/l for Lachnellula wilkommii and 4748 mgh/l for Leptographium wageneri. Although these values may be reached in the thin sapwood of Dahurian larch logs, they will probably not be approached in the heartwood of these logs using conventional tent fumigation techniques.
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