Graduate Thesis Or Dissertation

 

Production of monoclonal antibodies against infectious laryngotracheitis virus of chickens and their use in an indirect immunofluorescenct diagnostic test Public Deposited

Downloadable Content

Download PDF
https://ir.library.oregonstate.edu/concern/graduate_thesis_or_dissertations/6h440w39s

Descriptions

Attribute NameValues
Creator
Abstract
  • Monoclonal antibodies were developed against USDA challenge strain of infectious laryngotracheitis virus (ILTV). Indirect immunofluorescence test was used to detect antibodies in supernatants of hybridomas. Hybridoma cells were developed by fusing Sp 2/0 myeloma cells with spleen cells obtained from mice immunized four times with partially purified USDA challenge strain of infectious laryngotracheitis virus. The supernatant of three hybridomas, designated as 2D1D8, 2E11G2, 2C6C7 were found positive for antibody activity against USDA challenge strain of ILTV. Hybridomas producing antibodies were cloned by the limiting dilution method. All three monoclonal antibodies reacted with USDA challenge strain of ILTV, S 88 00224 strain of ILTV, and 86 1169 strain of ILTV in an indirect immunofluorescence test. None of the monoclonal antibodies reacted with avian adenovirus 301 or parrot herpes virus in an indirect immunofluorescence test. The monoclonal antibodies were isotyped, and all three monoclonal antibodies were found to be IgM.
Resource Type
Date Available
Date Copyright
Date Issued
Degree Level
Degree Name
Degree Field
Degree Grantor
Commencement Year
Advisor
Academic Affiliation
Non-Academic Affiliation
Subject
Rights Statement
Peer Reviewed
Language
Digitization Specifications
  • File scanned at 300 ppi (Monochrome) using Capture Perfect 3.0 on a Canon DR-9050C in PDF format. CVista PdfCompressor 4.0 was used for pdf compression and textual OCR.
Replaces
Additional Information
  • description.provenance : Approved for entry into archive by Patricia Black(patricia.black@oregonstate.edu) on 2013-01-03T18:36:40Z (GMT) No. of bitstreams: 1 AbbasFerhat1993.pdf: 3905975 bytes, checksum: 4b92f788bc2c72ae22deb0f7851a7b2f (MD5)
  • description.provenance : Submitted by Kirsten Clark (kcscannerosu@gmail.com) on 2013-01-02T18:42:35Z No. of bitstreams: 1 AbbasFerhat1993.pdf: 3905975 bytes, checksum: 4b92f788bc2c72ae22deb0f7851a7b2f (MD5)
  • description.provenance : Made available in DSpace on 2013-01-03T18:36:40Z (GMT). No. of bitstreams: 1 AbbasFerhat1993.pdf: 3905975 bytes, checksum: 4b92f788bc2c72ae22deb0f7851a7b2f (MD5) Previous issue date: 1992-10-28
  • description.provenance : Approved for entry into archive by Patricia Black(patricia.black@oregonstate.edu) on 2013-01-02T18:48:50Z (GMT) No. of bitstreams: 1 AbbasFerhat1993.pdf: 3905975 bytes, checksum: 4b92f788bc2c72ae22deb0f7851a7b2f (MD5)

Relationships

Parents:

This work has no parents.

In Collection:

Items