Graduate Thesis Or Dissertation
 

Guanosine tetrophosphate accumulation in Rhizobium meliloti

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https://ir.library.oregonstate.edu/concern/graduate_thesis_or_dissertations/6m311s323

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  • The effect of carbon source, nitrogen source, and amino acid starvation on the accumulation of guanosine tetraphosphate.and RNAand protein synthesis in Rhizobium meliloti are described in this report. An accumulation of ppGpp and a decrease in the rate of RNA and protein synthesis were observed in response to nitrogen source starvation. This nucleotide also accumulated when cells were starved for carbon or essential amino acids. RNA synthesis was inhibited during serine starvation, but upon methionine starvation, even though high levels of ppGpp were induced, no inhibition of RNA synthesis was observed. Based on these results, it was concluded that guanosine tetraphosphate is in fact synthesized in R. meliloti, and is probably involved in the regulatory response of this bacterium to carbon or nitrogen source limitation. Furthermore, it was apparent that ppGpp is not directly responsible for the inhibition of RNA synthesis during amino acid starvation.
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