Qualitative changes in cytoplasmic proteins in plants treated with plant growth regulating chemicals Public Deposited

http://ir.library.oregonstate.edu/concern/graduate_thesis_or_dissertations/6q182n65z

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  • This thesis concerns the alteration of the course of selective protein synthesis and morphogenesis in plants treated with growth regulating chemicals. Treatment of 48-hour-old "Alaska" pea seedlings (Pisum sativum L.) with 2, 4-D, IAA, NAA or picloram caused inhibition of epicotyl and primary root elongation and proliferation of massed lateral roots. Squash (Cucurbita maxima L.) and corn (Zea mays L.) seedlings also produced abnormal lateral roots after 2, 4-D treatment. The lateral roots induced by 2, 4-D in pea seedlings were initiated in the pericycle opposite all xylem poles over the length of the root between 6 and 9 hours after treatment. Lateral roots in control pea seedlings were also initiated in the pericycle but at a later time and were restricted to widely separated centers opposite a single xylem pole at a given point in the root. The soluble cytoplasmic proteins of plant root sections were fractionated by anionic gel electrophoresis in 10% acrylamide gels. Some proteins were common to all sections of pea roots but others varied quantitatively with position in the root. A few proteins were found only in certain portions of the root and may be associated with root development. Proteins with an Rf of 0.41 (band 0.41) were prominent members of the latter group. Corn and squash seedlings gave similar results including the prominent changes in the intensity of band 0.41. However, the electrophoretic patterns were markedly different among species. The electrophoretic patterns of proteins from roots of 2, 4-D-treated corn and squash seedlings and from roots of 2, 4-D-, IAA-, NAA-or picloram-treated pea seedlings differed quantitatively and qualitatively from patterns associated with untreated seedlings. Band 0.41 appeared in all sections and increased in intensity with distance from the root tip and time after treatment. In pea seedlings, band 0.41 was visible 12 hours after treatment with 2, 4-D. Pea seedlings preferentially incorporated alanine-C¹⁴ into proteins in band 0.41 between 6 and 12 hours after 2, 4-D treatment suggesting that synthesis of these proteins was initiated during this interval. The distribution of band 0.41 and the pattern and timing of lateral root development were clearly associated in primary root sections of control, 2, 6-D-and ethylene-treated pea seedlings. The close temporal and spatial association of band 0.41 with the initiation of lateral roots suggests a cause and effect relationship between these events. The identity of the proteins in band 0.41 is unknown, and there is no assurance band 0.41 from different root sections and species contains the same proteins. A procedure is described for the isolation of the proteins in band 0.41 by preparative scale gel electrophoresis.
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