Graduate Thesis Or Dissertation

 

Accumulation of dietary polychlorinated biphenyl by rainbow trout (Salmo gairdneri) Public Deposited

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  • This study was conducted to investigate the accumulation of dietary polychlorinated biphenyls (PCB's) by a Mt. Shasta strain of rainbow trout (Salmo gairdneri). A commercial mixture of PCB's (Aroclor 1254) was added to the diet at a level of 15 ppm on a dry weight basis. Accumulation of PCB's was measured using a gas-liquid chromatograph (GLC) equipped with an electron capture (EC) detector. The relative concentration of PCB's in the extractable lipid reached an equilibrium at 95 parts per million (ppm) after 8 weeks of dietary exposure. On a whole-fish basis, the concentration of PCB's increased with increases in the lipid content, which stabilized after 24 weeks to give the trout a concentration of 8.5 ppm PCB's. Absolute quantities (μg PCB/fish) increased as the fish grew, and after 32 weeks the trout contained an average of 636 μg PCB/fish. Total retention of PCB's from the diet after 32 weeks of exposure was 68%. PCB's were found to be distributed at a constant level in the lipid fraction of various tissues. However, due to the variation in lipid content of different tissues, large variation of PCB concentration on a whole-tissue basis existed. Elimination of PCB's from the trout was very slow if it occurred at all. Rainbow trout that were fed the PCB diet for 16 weeks and then the control diet for 16 weeks did not appear to eliminate any PCB residues during the latter period, although the relative concentration (ppm) decreased due to a simple dilution effect as the trout grew. Fish maintained on the PCB diet for 32 weeks and then starved for 8 weeks contained essentially the same quantity of PCB's before and after starvation. While the lipid content decreased by approximately one-half, the concentration of PCB's in the remaining lipid doubled. Peak patterns of PCB's isolated from the trout were identical to those of the Aroclor 1254 standard at all stages of the experiment, indicating that rainbow trout do not selectively absorb or metabolize PCB's. No differences were observed in the lipid content, growth, or size of the liver of fish on the PCB diet and control diet. Microscopic examination of liver tissues did not reveal any damage from ingestion of PCB's. No mortalities were attributed to PCB toxicity.
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