A selective medium for isolation of Bacteriodes nodosus Public Deposited

http://ir.library.oregonstate.edu/concern/graduate_thesis_or_dissertations/70795b51m

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  • The sensitivity of Bacteroides nodosus, causative agent of sheep foot rot, to 24 selected antimicrobial agents was tested. Many contaminants ordinarily associated with foot rot lesions were sensitive to lincomycin while B nodosus demonstrated resistance to this antibiotic. A concentration of 1.08 μg of lincomycin/ml of basal medium optimally inhibited contaminants while allowing growth of B nodosus. The basal medium was Eugon agar with 0.2% yeast extract and 10% defibrinated horse blood. Parallel inoculations of 31 foot rot lesion specimens onto basal medium and basal medium containing lincomycin (selective medium) was done. B nodosus was recovered from 16 of the specimens cultured on the selective medium and only three of the specimens cultured on the basal medium. The concentration of agar in the standard Eugon medium was found to influence the growth of B nodosus in the presence of lincomycin. Trimethoprim also exhibited potential selectivity for B nodosus. This is the first reported study in which an antimicrobial agent has been utilized in the selection for B nodosus from necrotic sheep foot rot material.
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