Functional domains of Agrobacterium tumefaciens single-stranded DNA binding protein VirE2 Public Deposited

http://ir.library.oregonstate.edu/concern/graduate_thesis_or_dissertations/70795c32m

Descriptions

Attribute NameValues
Creator
Abstract or Summary
  • Agrobacterium tumefaciens is a gram-negative soil bacterium that causes crown gall tumors on dicotyledenous plants. The transferred DNA (T-DNA) portion of the A. tumefaciens tumor-inducing (Ti) plasmid enters infected plant cells and integrates into plant nuclear DNA. The T-DNA is accompanied into plant cells by the VirD2 endonuclease covalently attached to its 5' end. VirE2, a cooperative, single-stranded DNA-binding protein is also transported into plant cells during infection by A. tumefaciens. VirD2 and VirE2 contain nuclear localization signals (NLSs) and are transported into the plant cell nucleus. The location of functional domains by the insertion of Xhol linker oligonucleotides throughout virE2 is reported. A ssDNA binding domain was located in the C-terminal half of VirE2, as well two domains involved in cooperative single-stranded DNA binding. Further, we isolated a mutation in the central region of VirE2 that decreased tumorigenicity, but did not affect ssDNA binding.
Resource Type
Date Available
Date Copyright
Date Issued
Degree Level
Degree Name
Degree Field
Degree Grantor
Commencement Year
Advisor
Committee Member
Academic Affiliation
Non-Academic Affiliation
Subject
Rights Statement
Peer Reviewed
Language
Digitization Specifications
  • File scanned at 300 ppi (Monochrome) using Capture Perfect 3.0 on a Canon DR-9050C in PDF format. CVista PdfCompressor 4.0 was used for pdf compression and textual OCR.
Replaces
Additional Information
  • description.provenance : Approved for entry into archive by Patricia Black(patricia.black@oregonstate.edu) on 2012-10-08T18:46:29Z (GMT) No. of bitstreams: 1 DombekPriscillaEmily1997.pdf: 9064580 bytes, checksum: 79d6a37e0eabca76d7411f2a099bf0ce (MD5)
  • description.provenance : Made available in DSpace on 2012-10-08T18:49:11Z (GMT). No. of bitstreams: 1 DombekPriscillaEmily1997.pdf: 9064580 bytes, checksum: 79d6a37e0eabca76d7411f2a099bf0ce (MD5) Previous issue date: 1996-07-18
  • description.provenance : Approved for entry into archive by Patricia Black(patricia.black@oregonstate.edu) on 2012-10-08T18:49:11Z (GMT) No. of bitstreams: 1 DombekPriscillaEmily1997.pdf: 9064580 bytes, checksum: 79d6a37e0eabca76d7411f2a099bf0ce (MD5)
  • description.provenance : Submitted by Kirsten Clark (kcscannerosu@gmail.com) on 2012-10-08T17:25:34Z No. of bitstreams: 1 DombekPriscillaEmily1997.pdf: 9064580 bytes, checksum: 79d6a37e0eabca76d7411f2a099bf0ce (MD5)

Relationships

In Administrative Set:
Last modified: 08/20/2017

Downloadable Content

Download PDF
Citations:

EndNote | Zotero | Mendeley

Items