Graduate Thesis Or Dissertation
 

The effect of variation of some physical and chemical factors on the rate of growth of Botyrococcus braunii (Chlorophyta, Chlorococcales)

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  • The culture of the green alga Botryococcus braunii Kutz. obtained for use in this study was contaminated with bacteria and fungi. Algal colonies from this culture sonicated, rinsed and treated with penicillin (500 μg/ml), gentamicin sulfate (50 μg/ml) and amphotericin B (5 μg/ml) were found to be axenic when incubated in ten microbiological media. The density of the cultures was determined as the dry weight of the alga. The loss of hydrocarbon during this procedure was examined and small losses were found. The minimum size of algal sample needed to accurately determine the density of a culture was estimated to be 0.16 mg of the alga. The increase in density of the axenic B. braunii culture grown in a modified Chu no. 10 medium over an extended period of time was monitored. Logarithmic growth was observed for the first 15 days with an approximate 3-day generation time. The rate of growth of the alga decreased greatly after 20 days. The effect of media containing various concentrations of nitrogen on the growth of the alga was observed. The rate of growth of the alga increased with increasing concentrations of nitrogen up to 6.6 p.p.m. Above this concentration no further increase or decrease in the rate of growth was observed. The effect on growth of varying concentrations of phosphorus was investigated and a relatively small influence was observed with the concentrations of phosphorus used. Two experiments were performed on the effect of varying the H⁺-ion concentration on the growth of the B. braunii. The alga was observed to grow at a rate relatively unaffected by variation of H⁺-ion concentrations ranging from pH 5.5 to 9.0. At a pH above or below this range there appeared to be a decrease in rate of growth. The effect on growth of various temperatures was also investigated. The rate of growth of the alga at 12 C and 25 C was relatively similar. At 29 C no increase was observed.
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