Non-random labelling of geraniol biosynthesized from ¹⁴Co₂ in Pelargonium graveolens Public Deposited

http://ir.library.oregonstate.edu/concern/graduate_thesis_or_dissertations/736668929

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  • The acyclic monoterpene geraniol was biosynthesized from ¹⁴CO₂ in Pelargonium graveolens (rose geranium) and isolated from the hexane-extractable fraction in five experiments. Experiments 1 and 2 were preliminary experiments in which the total incorporation of label into geranium oil was determined. In experiments 3 and 4a and 4b both incorporation and distribution of ¹⁴C in geraniol were determined. Radioactive geraniol from experiments 3 and 4a and 4b was degraded by ozonolysis. Radioactivity of the terminal three carbons (C-7, 8 and 10), which presumably represents 3/5 of that portion of the geraniol molecule derived from 3, 3-dimethylallyl pyrophosphate (DMAPP),was determined. In early samples (two hours) there was some apparent preferential labelling in the DMAPP moiety of geraniol. In longer time, as the level of label in geraniol increased, the isopentenyl pyrophosphate (IPP) portion became preferentially labelled. This trend was observed consistently. The presence of a metabolic pool of DMAPP in the plant is suggested as one factor in explaining these results. Further possible factors are proposed, based on the results, which include compartmentation of IPP and DMAPP metabolism, alternative pathways to geraniol, and multiple sites of geraniol biosynthesis.
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