Graduate Thesis Or Dissertation
 

Neorickettsia helminthoeca in cell culture

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https://ir.library.oregonstate.edu/concern/graduate_thesis_or_dissertations/7m01bp17n

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  • The etiologic agent of salmon poisoning disease was found to be Neorickettsia helminthoeca, although a second organism, the Elokomin fluke fever agent, may also be involved in other areas. Primary cultures of dog leucocytes were found to support the in vitro cultivation of Neorickettsia helminthoeca as were canine sarcoma 503 cells and mouse lymphoblasts MB III. Quantitative methods were not applied to in vitro studies of multiplication of the rickettsia because it failed to grow sufficiently in laboratory animals or in chicken embryo yolk sacs to do so. By ultrastructural analysis it was learned that Neorickettsia helminthoeca was structurally similar to other rickettsiae. Rickettsial cells were seen as circular profiles or rod-shaped cells 0. 5μ wide and up to 0.7μ. long. They were bounded by a cell wall and an underlying cytoplasmic membrane. Each of the membranes was a trilayered structure and showed the unit membrane structure. Koch's postulates were fulfilled as completely as possible for an organism that is an obligate intracellular parasite. Neorickettsia helminthoeca was found in all cases of salmon poisoning disease. The rickettsia was isolated and grown in culture. The isolated culture was found to reproduce the disease when inoculated into susceptible dogs. The rickettsia was observed in and recovered from the inoculated dog. Neorickettsia helminthoeca in cell systems can be used as a model to further investigate the host-parasite relationship.
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