Development of a method to elucidate biodegradation pathways of chlorinated one and two carbon compounds using a gas-permeable-membrane-supported methylotrophic biofilm Public Deposited

http://ir.library.oregonstate.edu/concern/graduate_thesis_or_dissertations/7s75dg46t

Descriptions

Attribute NameValues
Creator
Abstract or Summary
  • A gas-permeable-membrane-supported (GPMS) biofilm consisting of methylotrophic bacteria was effective in degrading chlorinated methanes, ethanes, and ethenes. The biofilm was developed on a gas-permeable fabric (Goretex, W.L.Gore & Associates, Elkton, Maryland) that divided a reactor vessel into a liquid compartment and a gas compartment. Goretex is a nylon-backed teflon mesh that allows gas transfer, but is impermeable to water. Methane and oxygen were diffused from a gas compartment, through the membrane, to the methylotrophic biofilm on the liquid side of the membrane. During the biofilm's development, methane served as the sole carbon source and electron donor, and oxygen served as the electron acceptor. Inorganic nutrients were supplied in the bulk aqueous solution. The chlorinated compounds were added to the bulk liquid. Removal of the compounds was monitored and the methylotrophic GPMS biofilm was shown to be effective in degrading dichloromethane, 1,2-dichloroethane; and cis 1,2-dichloroethene. Rates of degradation increased in the order of chlorinated ethene, ethane, and methane. The GPMS system can be operated in either batch or continuous flow mode with similar degradation rates resulting from each process. A model was developed to predict metabolic product concentration as a function of time and retention time in batch or continuous flow reactors. No metabolic products were detected, but it is apparent that the degradation rates of any metabolites of dichloromethane or 1,2-dichloroethane are probably at least ten times greater than that of the original parent compounds.
Resource Type
Date Available
Date Copyright
Date Issued
Degree Level
Degree Name
Degree Field
Degree Grantor
Commencement Year
Advisor
Academic Affiliation
Non-Academic Affiliation
Subject
Rights Statement
Peer Reviewed
Language
Digitization Specifications
  • File scanned at 300 ppi (Monochrome) using Capture Perfect 3.0.82 on a Canon DR-9080C in PDF format. CVista PdfCompressor 4.0 was used for pdf compression and textual OCR.
Replaces
Additional Information
  • description.provenance : Approved for entry into archive by Patricia Black(patricia.black@oregonstate.edu) on 2013-05-29T18:53:15Z (GMT) No. of bitstreams: 1 RyanKellyM1988.pdf: 828169 bytes, checksum: ddbf2c0038663885e17d20c4e1a61498 (MD5)
  • description.provenance : Made available in DSpace on 2013-06-17T23:18:16Z (GMT). No. of bitstreams: 1 RyanKellyM1988.pdf: 828169 bytes, checksum: ddbf2c0038663885e17d20c4e1a61498 (MD5) Previous issue date: 1988-04-08
  • description.provenance : Submitted by Kim Stowell (ksscannerosu@gmail.com) on 2013-05-28T23:00:20Z No. of bitstreams: 1 RyanKellyM1988.pdf: 828169 bytes, checksum: ddbf2c0038663885e17d20c4e1a61498 (MD5)
  • description.provenance : Approved for entry into archive by Deborah Campbell(deborah.campbell@oregonstate.edu) on 2013-06-17T23:18:16Z (GMT) No. of bitstreams: 1 RyanKellyM1988.pdf: 828169 bytes, checksum: ddbf2c0038663885e17d20c4e1a61498 (MD5)

Relationships

In Administrative Set:
Last modified: 08/16/2017

Downloadable Content

Download PDF
Citations:

EndNote | Zotero | Mendeley

Items