Graduate Thesis Or Dissertation
 

Development of a method to elucidate biodegradation pathways of chlorinated one and two carbon compounds using a gas-permeable-membrane-supported methylotrophic biofilm

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https://ir.library.oregonstate.edu/concern/graduate_thesis_or_dissertations/7s75dg46t

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  • A gas-permeable-membrane-supported (GPMS) biofilm consisting of methylotrophic bacteria was effective in degrading chlorinated methanes, ethanes, and ethenes. The biofilm was developed on a gas-permeable fabric (Goretex, W.L.Gore & Associates, Elkton, Maryland) that divided a reactor vessel into a liquid compartment and a gas compartment. Goretex is a nylon-backed teflon mesh that allows gas transfer, but is impermeable to water. Methane and oxygen were diffused from a gas compartment, through the membrane, to the methylotrophic biofilm on the liquid side of the membrane. During the biofilm's development, methane served as the sole carbon source and electron donor, and oxygen served as the electron acceptor. Inorganic nutrients were supplied in the bulk aqueous solution. The chlorinated compounds were added to the bulk liquid. Removal of the compounds was monitored and the methylotrophic GPMS biofilm was shown to be effective in degrading dichloromethane, 1,2-dichloroethane; and cis 1,2-dichloroethene. Rates of degradation increased in the order of chlorinated ethene, ethane, and methane. The GPMS system can be operated in either batch or continuous flow mode with similar degradation rates resulting from each process. A model was developed to predict metabolic product concentration as a function of time and retention time in batch or continuous flow reactors. No metabolic products were detected, but it is apparent that the degradation rates of any metabolites of dichloromethane or 1,2-dichloroethane are probably at least ten times greater than that of the original parent compounds.
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  • File scanned at 300 ppi (Monochrome) using Capture Perfect 3.0.82 on a Canon DR-9080C in PDF format. CVista PdfCompressor 4.0 was used for pdf compression and textual OCR.
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