Graduate Thesis Or Dissertation
 

Preliminary biochemical evidence for an ascospore coat protein in Saccharomyces cerevisiae : partial characterization and timing of synthesis

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  • Sporulation in the yeast Saccharomyces cerevisiae involves the process of meiosis which is accompanied by ascospore formation. Using intact cells or spores, preliminary biochemical evidence is given to identify ascospore wall components not found in vegetative cell walls. Cell surface components are solubilized by treatment with 8 M urea and subsequently dialyzed to precipitate hydrophobic components. These urea-soluble hydrophobic components were analyzed by SDS-polyacrylamide gel electrophoresis. The spore wall component not present in vegetative cell wall extracts appears to be proteinaceous and possesses no detectable carbohydrate moiety as determined by periodic acid-Schiff reagent (PAS) staining procedures. Further experiments indicate that a component of snail gut enzyme, which is used to liberate ascospores from asci, co-migrates with the spore wall protein. Due to the hydrophobic nature of the ascospore wall, there is evidence suggestive of some cytoplasmic binding to cell wall extracts. It is unclear as to whether or not the ascospore wall protein is sporulation-specific. Pulse-labeling experiments indicate that the spore coat component is synthesized prior to ascospore formation as early as zero to four hours after inoculation into sporulation medium.
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