Graduate Thesis Or Dissertation
 

Physiological and hematological changes in chum salmon (Oncorhynchus keta) infected with erythrocytic necrosis virus

Public Deposited

Downloadable Content

Download PDF
https://ir.library.oregonstate.edu/concern/graduate_thesis_or_dissertations/8910jx24x

Descriptions

Attribute NameValues
Creator
Abstract
  • Chum salmon (Oncorhynchus keta) were artificially infected with erythrocytic necrosis virus (ENV) to study the physiological and hematolgical consequences of ENV-infection. Infected and control fish were held in pathogen-free seawater and sampled weekly for five weeks. Physiological tests included plasma cortisol, glucose, protein, and osmolality, blood lactic acid, and liver glycogen concentrations. In general, fish infected with ENV had higher liver glycogen values, and lower plasma glucose and blood lactic acid levels than control fish. Hematological tests included red and white blood cell (RBC and WBC) counts, hematocrits, blood hemoglobin concentrations, and erythrocyte fragility. Infected fish had lower RBC counts, hematocrits, and hemoglobin concentrations, higher WBC counts, and more fragile erythrocytes than control fish. Other blood parameters calculated from the hematology data indicated that the erythrocytes of infected fish had higher mean corpuscular volumes, depressed mean corpuscular hemoglobin concentrations, and slightly lower mean corpuscular hemoglobin. Erythrocytic inclusions were observed in the cytoplasm of RBC of infected fish, and unnucleated cells were observed by week 2. In this experiment, severity of infection progressed steadily through week 4, after which the fish appeared to be recovering. In a second study, fish were infected with ENV, held for three weeks, and recovery from exercise was measured. Plasma glucose and osmolality were higher in infected fish, while plasma cortisol and blood lactate levels were only slightly elevated. These studies indicate that chum salmon were able to withstand the effects of ENVinfection without irreversible physiological consequences. However, when subjected to-exercise, infected fish recovered more slowly than controls and seemed to have increased osmoregulatory difficulties.
Resource Type
Date Available
Date Issued
Degree Level
Degree Name
Degree Field
Degree Grantor
Commencement Year
Advisor
Academic Affiliation
Non-Academic Affiliation
Subject
Rights Statement
Publisher
Peer Reviewed
Language
Digitization Specifications
  • File scanned at 300 ppi (Monochrome) using ScandAll PRO 1.8.1 on a Fi-6770A in PDF format. CVista PdfCompressor 5.0 was used for pdf compression and textual OCR.
Replaces

Relationships

Parents:

This work has no parents.

In Collection:

Items

Thumbnail Title Date Uploaded Visibility Actions
file details HaneyDennisC1986.pdf 2017-08-10 Public Download