Regulation of the terminal reactions for methionine biosynthesis in yeast Public Deposited

http://ir.library.oregonstate.edu/concern/graduate_thesis_or_dissertations/8g84mp70j

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  • Studies were carried out to elucidate the mechanism of enzymatic control of methionine biosynthesis in Saccharomyces cerevisiae. Enzymatic studies demonstrated that, in addition to the methionine activating enzyme, the S-adenosylmethionine:homocysteine transmethylase enzyme was induced during cultivation in the presence of excess methionine. A similar, though reduced effect was observed when homocysteine and serine were present in the medium. The serine hydroxymethylase enzyme was repressed under these conditions. Ethionine-resistant mutants were isolated to facilitate these studies. These organisms were shown to possess a reduced ability for converting methionine to S-adenosylmethionine. These organisms accumulated more intracellular methionine than the wild-type strain. The mutants were also found to excrete more methionine into the medium than the parental strain. All mutants tested showed a reduced level of the transmethylase enzyme. Two showed a reduced level of the methionine activating enzyme. Studies which measured the accumulation and intracellular distribution of ethionine (ethyl-C¹⁴) and methionine (methyl-C¹⁴) were also carried out. The mutants were able to exclude' the analog from participation in most cellular processes.
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  • File scanned at 300 ppi using Capture Perfect 3.0 on a Canon DR-9050C in PDF format. CVista PdfCompressor 5.0 was used for pdf compression and textual OCR.
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