Control of macromolecular synthesis in Saccharomyces cerevisiae Public Deposited

http://ir.library.oregonstate.edu/concern/graduate_thesis_or_dissertations/8p58ph57z

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  • Macromolecular synthesis in Saccharomyces cerevisiae
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  • Synthesis of total RNA, DNA, and protein was followed in a haploid yeast auxotroph during starvation for required amino acids and uracil, and during shift-up and shift-down conditions. During amino acid starvation, synthesis of macromolecular constituents was not immediately affected, reflecting the presence of large intracellular amino acid pools. Under conditions in which there was no net RNA and protein synthesis (amino acid and uracil starvation), total DNA continued to be synthesized for a total increase of 10-15%. The results suggested that cells engaged in DNA synthesis at the introduction of starvation conditions complete replication of the genome but do not initiate new periods of DNA synthesis. Upon return of a starved culture to complete medium, a period of DNA synthesis was required before the culture began to divide at the rate characteristic for complete medium. Compounds which are known to inhibit DNA synthesis in bacteria had no effect upon starved yeast cultures returned to complete medium. Under shift-down conditions, cultures exhibited a lag in response to that observed during amino acid starvation. During shift-up conditions, DNA and protein increased in a stepwise fashion before assuming a continuous rate of increase characteristic of the new medium. During shift-up RNA was synthesized initially at a high rate; this was found not to be due to exclusive synthesis of a particular class of RNA.
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