Graduate Thesis Or Dissertation
 

The effect of streptomycin on proline synthesis in Escherichia coli

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  • The effect of streptomycin on an early reaction of proline synthesis, the production of glutamic-γ-semialdehyde, in resting cell suspensions of antibiotic-sensitive, resistant, and dependent strains of Escherichia coli was examined, and the effect on glutamic-γ-semialdehyde production was found to correspond to the growth response of the organisms to streptomycin. Inhibition of glutamic-γ-semialdehyde production in sensitive strains was found not to be caused by streptomycin-induced changes in permeability to substrate or product of the reaction. The effects of pH, ionic strength, and the presence of purines, pyrimidines, and nucleotides on streptomycin action were examined, and possibilities for the mode and site of streptomycin action are discussed.
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  • File scanned at 300 ppi using Capture Perfect 3.0 on a Canon DR-9050C in PDF format. CVista PdfCompressor 5.0 was used for pdf compression and textual OCR.
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