Studies on viruses isolated from roses with rose mosaic symptoms Public Deposited

http://ir.library.oregonstate.edu/concern/graduate_thesis_or_dissertations/9g54xm452

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  • Previous work on the rose mosaic virus has produced uncertainty concerning the identity and properties of this virus. This work was undertaken to help establish the true identity of the virus and to determine some of its properties. A mechanically transmissible virus associated with rose mosaic virus disease of Rosa dilecta, var. Red Delight was purified by homogenizing infected tissue in pH 9.5 borate buffer containing thioglycollic acid and activated charcoal, clarifying the filtrate with ether- CCl₄ and concentrating the suspension by alternate high and low speed and density gradient centrifugation. The ultraviolet absorption of the purified virus preparation was measured and the percent RNA and protein were calculated as 24.8 and 75.2 percent respectively. The virus suspension consisted of spherical particles, as determined by electron microscopy, with two size ranges at 20-30 mμ, and 35-45 mμ. An antiserum to the virus was prepared. Attempts were made to determine the serological relationship of the virus to other known viruses, but the results were not conclusive.
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