The mineralogy and morphology of loess derived soils along a traverse in northeastern Oregon Public Deposited

http://ir.library.oregonstate.edu/concern/graduate_thesis_or_dissertations/9k41zh25t

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  • The lentil strain of pea seedborne mosaic virus, PSbMV-L, was distinguished from the standard isolates of PSbMV by a natural inoculum reservoir in lentil seed and by separate resistance genes in Pisum and Lens. PSbMV-L was intermediate in symptom severity between the standard and New Zealand isolates of PSbMV. Seed transmission rates up to 16.7% were observed for PSbMV-L in seed increase plots of infected lentil accessions, but the virus was not detected in commercial lentil plantings. The virus was nonpersistently transmitted by Acyrthosiphon pisum. Maximum rates of aphid transmission occurred with acquisition access periods of 3 to 5 minutes and ranged from 80 to 100%. Aphid transmission rates decreased with increasing temperature. Apparent trimodal transmission of PSbMV-L was observed. PSbMV-L is characterized by intrinsic particle instability. Partial purification was obtained by extracting infected lentil leaves in a neutral buffer, clarification with 7.5% chloroform, centrifugation through PEG (6 %)- sucrose (35%) cushions and equilibrium centrifugation in CsC1 gradients. The absorbance ratio 260/280 was 1.15. PSbMV-SL25 and PSbMV-L were indistinguishable on the basis of protein subunit molecular weight and could not be differentiated using classical serological techniques. The strains could be separated by quantitative ELISA using polyclonal sera produced in rabbit or chicken. Attempts to achieve greater antibody specificity were made with spleen cells from previously immunized mice that were fused with myoloma cells to create antibody-secreting hybridomas. The resultant colonies were screened using a modified ELISA against PSbMV-SL25 and PSbMV-L for the presence of specific antibody production. Selected colonies were expanded. The molecular weight of PSbMV-L RNA was estimated at 3.5 million. DNA complementary to PSbMV-C4 RNA was prepared using the random primer method of Taylor et al. Hybridization kinetics with PSbMV-L, PSbMV-SL25 and BYMV-Gil RNA were studied using Sl-nuclease assays. The lentil strain showed significantly lower levels of hybridization than the homologous control. Heterologous hybridization was severely decreased when the stringency of the assay conditions was increased.
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