Development of monoclonal antibodies against the myxosporean, ceratomyxa shasta, and characterization of the salmonid host response Public Deposited

http://ir.library.oregonstate.edu/concern/graduate_thesis_or_dissertations/9s161862n

Descriptions

Attribute NameValues
Creator
Abstract or Summary
  • Monoclonal antibodies (Mabs) were produced against the myxosporean parasite, Ceratomyxa shasta, and were characterized by indirect immunofluorescent techniques, western blot analysis, and immunoelectron microscopy. The resulting Mabs reacted specifically with the trophozoite and sporoblast stages of the parasite, but not with the mature spore, indicating that certain antigens are stage-specific. Two of the Mabs produced also reacted with trout white blood cells as evidenced by indirect fluorescent antibody techniques (IFAT) and Western blot analysis. Further characterization of these cross-reacting antibodies showed that they were directed against carbohydrate epitopes on both the parasite and trout immunoglobulin heavy chain. One hybridoma produced antibodies of high specificity for prespore stages of C. shasta. Ultrastructural analysis of the parasite using immunogold labeling showed that the Mab specifically bound antigen located in the cytoplasm of the trophozoites. The C. shasta-specific antibodies produced by monoclonal technology, standard histological methods, and scanning electron microscopy were used to investigate the host response of Sa lmo gairdneri against infection by the parasite. In infected fish the parasite was first observed in the mucosal epithelium of the posterior intestine using IFAT. As the trophozoites proliferated, the infection spread anteriorally and into the submucosa, muscularis, and serosa of the intestine. Hepatic tissues were also infected early in the disease process. In the terminal stages of the infection, trophozoites had penetrated the stomach, pyloric caeca, pancreas, and adipose tissues and were also observed in the blood and kidney. In fish refractory to infection, the parasite was observed in the lumen of the intestine. Examination of infection in a moribound fish by scanning electron microscopy showed extensive destruction of the mucosal folds of the posterior intestine. Although a vigorous tissue response was evident in infected tissues, trout antibodies specific for the parasite were not detected by either Western blot analysis or IFAT. The distribution of the infective stage of C. shasta in the Columbia River basin was also investigated. The range of the parasite was extended in the Columbia River to its confluence with the Snake River and in the Snake River to Oxbow Dam.
Resource Type
Date Available
Date Copyright
Date Issued
Degree Level
Degree Name
Degree Field
Degree Grantor
Commencement Year
Advisor
Academic Affiliation
Non-Academic Affiliation
Subject
Rights Statement
Peer Reviewed
Language
Digitization Specifications
  • File scanned at 300 ppi (Monochrome, 8-bit Grayscale) using ScandAll PRO 1.8.1 on a Fi-6770A in PDF format. CVista PdfCompressor 5.0 was used for pdf compression and textual OCR.
Replaces
Additional Information
  • description.provenance : Submitted by Kaylee Patterson (kdpscanner@gmail.com) on 2013-05-16T21:27:50Z No. of bitstreams: 1 BartholomewJerriLee1989.pdf: 3697055 bytes, checksum: f4812e36df959f6bb48560b2f5aaf8e6 (MD5)
  • description.provenance : Approved for entry into archive by Patricia Black(patricia.black@oregonstate.edu) on 2013-05-29T15:51:46Z (GMT) No. of bitstreams: 1 BartholomewJerriLee1989.pdf: 3697055 bytes, checksum: f4812e36df959f6bb48560b2f5aaf8e6 (MD5)
  • description.provenance : Made available in DSpace on 2013-07-16T21:01:06Z (GMT). No. of bitstreams: 1 BartholomewJerriLee1989.pdf: 3697055 bytes, checksum: f4812e36df959f6bb48560b2f5aaf8e6 (MD5) Previous issue date: 1988-11-11
  • description.provenance : Approved for entry into archive by Patricia Black(patricia.black@oregonstate.edu) on 2013-07-16T21:01:06Z (GMT) No. of bitstreams: 1 BartholomewJerriLee1989.pdf: 3697055 bytes, checksum: f4812e36df959f6bb48560b2f5aaf8e6 (MD5)

Relationships

In Administrative Set:
Last modified: 08/04/2017

Downloadable Content

Download PDF
Citations:

EndNote | Zotero | Mendeley

Items