Antigenic comparison of five bovine adenovirus type 3 isolates using a fluorescent foci assay system Public Deposited

http://ir.library.oregonstate.edu/concern/graduate_thesis_or_dissertations/b8515r29x

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  • Four bovine adenovirus type 3 isolates, recovered from different areas in Oregon and Washington, were compared antigenically with the original prototype strain which was isolated in England. The fluorescent foci assay method was used to quantitatively establish viral titers. A serum-viral neutralization test was performed with a standard dose of each virus and the 50% fluorescent foci neutralization level of antiserum was calculated. The assay system was shown to be valid as viral titrations showed that a linear relationship existed between virus concentrations (represented by the log dilution of the virus) and number of infected cells (represented by the number of fluorescent foci). Viral strain 5C was shown to be identical antigenically to the WBR 1 prototype virus. Isolate 126N and 837C varied in their antigenic properties but this variation was not believed to be great enough to warrant their designation as separate subtype strains. Isolate 62N was shown to vary significantly from the WBR 1 strain, this isolate should be considered as a subtype of the WBR 1 prototype strain.
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  • description.provenance : Approved for entry into archive by Patricia Black(patricia.black@oregonstate.edu) on 2013-10-15T20:08:55Z (GMT) No. of bitstreams: 1 LiaumHardiKianHong1978.pdf: 2081835 bytes, checksum: 1f7a6a1dfec12234551ca0019b6122f4 (MD5)
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