Cytokinin production by species of the fungus Taphrina Public Deposited

http://ir.library.oregonstate.edu/concern/graduate_thesis_or_dissertations/bn999954j

Descriptions

Attribute NameValues
Creator
Abstract or Summary
  • Chemically defined liquid media were devised which supported excellent growth of Taphrina cerasi and T. deformans. When grown in fermentation culture vessels, T. cerasi and T. deformans released cytokinins into the liquid media. The presence of these cell division factors was demonstrated with the soybean tissue assay which is specific for the detection of cytokinins. Paper chromatography in four solvent systems revealed that T. cerasi produced compounds which have migration patterns similar to zeatin, zeatin riboside, N⁶-(Δ²-isopentenyl)adenine (2iP), and N⁶-(Δ²-isopentenyl)adenosine (2iPA) in each of the solvent systems. T. deformans produced a zeatin-like compound, a cytokinin with chromatographic properties similar to 2iP, and a third cytokinin whose migration pattern did not correspond to any of the common cytokinin standards. The soybean tissue assay was used to calculate the concentration of cytokinin released by the fungi into the culture media. The amount of cytokinin produced by T. cerasi was calculated to be approximately 45 μ.g KE/liter of medium, while the amount released by T. deformans was found to be about 75 μg KE/liter. Insoluble polyvinylpyrrolidone was effective in the partial purification of cytokinin-like substances in extracts of the fungal liquid culture medium. Cytokinins or cytokinins in combination with auxin applied to healthy peach leaves did not mimic the disease symptoms. Attempts to induce mycelial growth of species of Taphrina were unsuccessful.
Resource Type
Date Available
Date Copyright
Date Issued
Degree Level
Degree Name
Degree Field
Degree Grantor
Commencement Year
Advisor
Academic Affiliation
Non-Academic Affiliation
Subject
Rights Statement
Peer Reviewed
Language
Digitization Specifications
  • File scanned at 300 ppi (Monochrome, 8-bit Grayscale, 24-bit Color) using ScandAll PRO 1.8.1 on a Fi-6670 in PDF format. CVista PdfCompressor 5.0 was used for pdf compression and textual OCR.
Replaces
Additional Information
  • description.provenance : Approved for entry into archive by Patricia Black(patricia.black@oregonstate.edu) on 2013-12-02T16:31:45Z (GMT) No. of bitstreams: 1 JohnstonJamesC1974.pdf: 1717688 bytes, checksum: cb721a727e0013c2486d9d117b91a554 (MD5)
  • description.provenance : Approved for entry into archive by Kirsten Clark(kcscannerosu@gmail.com) on 2013-12-03T21:11:14Z (GMT) No. of bitstreams: 1 JohnstonJamesC1974.pdf: 1717688 bytes, checksum: cb721a727e0013c2486d9d117b91a554 (MD5)
  • description.provenance : Submitted by Madison Medley (mmscannerosu@gmail.com) on 2013-11-26T19:44:32Z No. of bitstreams: 1 JohnstonJamesC1974.pdf: 1717688 bytes, checksum: cb721a727e0013c2486d9d117b91a554 (MD5)
  • description.provenance : Made available in DSpace on 2013-12-03T21:11:14Z (GMT). No. of bitstreams: 1 JohnstonJamesC1974.pdf: 1717688 bytes, checksum: cb721a727e0013c2486d9d117b91a554 (MD5) Previous issue date: 1973-08-09

Relationships

In Administrative Set:
Last modified: 08/14/2017

Downloadable Content

Download PDF
Citations:

EndNote | Zotero | Mendeley

Items