Graduate Thesis Or Dissertation
 

Cytokinin production by species of the fungus Taphrina

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  • Chemically defined liquid media were devised which supported excellent growth of Taphrina cerasi and T. deformans. When grown in fermentation culture vessels, T. cerasi and T. deformans released cytokinins into the liquid media. The presence of these cell division factors was demonstrated with the soybean tissue assay which is specific for the detection of cytokinins. Paper chromatography in four solvent systems revealed that T. cerasi produced compounds which have migration patterns similar to zeatin, zeatin riboside, N⁶-(Δ²-isopentenyl)adenine (2iP), and N⁶-(Δ²-isopentenyl)adenosine (2iPA) in each of the solvent systems. T. deformans produced a zeatin-like compound, a cytokinin with chromatographic properties similar to 2iP, and a third cytokinin whose migration pattern did not correspond to any of the common cytokinin standards. The soybean tissue assay was used to calculate the concentration of cytokinin released by the fungi into the culture media. The amount of cytokinin produced by T. cerasi was calculated to be approximately 45 μ.g KE/liter of medium, while the amount released by T. deformans was found to be about 75 μg KE/liter. Insoluble polyvinylpyrrolidone was effective in the partial purification of cytokinin-like substances in extracts of the fungal liquid culture medium. Cytokinins or cytokinins in combination with auxin applied to healthy peach leaves did not mimic the disease symptoms. Attempts to induce mycelial growth of species of Taphrina were unsuccessful.
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