Enzymatic patterns of ionocytes in Oncorhynchus tshawytscha Public Deposited

http://ir.library.oregonstate.edu/concern/graduate_thesis_or_dissertations/br86b616c

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  • Ionocytes, Enzymatic patterns
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  • Characterization of the enzymatic activity of the ionocyte (mitochondria-rich cell) in the gill epithelium of Oncorhynchus tshawytscha was explored. 1. A method for estimating the enzymatic activity per cell for tissues is described. 2. The DNA content per cell for nucleated erythrocytes (O. tshawytscha) was determined to be 9.47 x 10⁻¹² gms. 3. The total DNA content of gill and liver tissue was determined for fish of various sizes and the cell populations per gm of tissue were calculated. 4. Experimental requirements for the enzyme assay of gill and liver mitochondrial fractions are described. 5. A threefold increase in gill succinate-cytochrome c reductase activity was found for gill epithelium functioning in the marine environment compared to the freshwater environment whereas there was no change in liver succinate-cytochrome c reductase levels.
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