Graduate Thesis Or Dissertation
 

A comparison of radiation induced lipid changes in Micrococcus radiodurans, Sarcina lutea, and Pseudomonas aeruginosa

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  • Quantity variations in pigments and lipid classes were determined following γ-irradiation of Micrococcus radiodurans, Sarcina lutea, and Pseudomonas aeruginosa. Pigmentation was determined by extraction with organic solvents and measured as optical density at 475 mμ for M. radiodurans and S. lutea, and 410 mμ for Ps. E aetuginosa. Pigments of M. radiodurans and S. lutea were inactivated at a linear rate with increased doses. The non-carotenoid pigment of Ps. aeruginosa was not significantly affected at a maximum dose of 2,040 krads. Quantitation of neutral lipids and neutral lipid hydrolysates was accomplished with thin layer chromatography. The lipid classes involved were separated on thin layer plates, charred with concentrated H₂SO₄ and heating, and densitometric determinations made. No differences were observed in the lipid classes of M. radiodurans and S. lutea following doses up to 2, 040 krads. A dose of 2, 040 krads resulted in a large increase in free fatty acids of Ps. aeruginosa.
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