Sterol 24(28) methylene reductase in Saccharomyces cerevisiae

Graduate Thesis Or Dissertation

Public Deposited

Citeable URL: https://ir.library.oregonstate.edu/concern/graduate_thesis_or_dissertations/cn69m7844

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Attribute Name	Values
Creator	Neal, William Daniel
Abstract	A cell free extract from Saccharomyces cerevisiae containing the enzyme that causes reduction of the 24(28)methylene group on the side chain of ergosterol has been studied. Optimal conditions for the 24(28)methylene reductase were obtained. The enzyme activity was increased in cells grown with ethanol as the substrate. The Km was calculated as 10 μM. Within the limits of the assay, enzymatic activity was not present in ergostatetraene accumulating mutants that were tested. The enzyme was competitively inhibited when tested against a naturally occurring azasterol. The dissociation constant (KI) was calculated as 17 μM. The enzyme reaction is not reversible under the conditions provided by the assay.
Resource Type	Masters Thesis
Date Available	2013-11-01T20:07:05+00:00
Date Issued	1977-05-05
Degree Level	Master's
Degree Name	Master of Science (M.S.)
Degree Field	Microbiology
Degree Grantor	Oregon State University
Commencement Year	1977
Advisor	Parks, Leo W.
Academic Affiliation	Microbiology
Non-Academic Affiliation	Oregon State University. Graduate School
Subject	Reduction (Chemistry)
Rights Statement	Copyright Not Evaluated
Publisher	Oregon State University
Peer Reviewed	No
Language	English [eng]
Digitization Specifications	File scanned at 300 ppi (Monochrome) using ScandAll PRO 1.8.1 on a Fi-6770A in PDF format. CVista PdfCompressor 5.0 was used for pdf compression and textual OCR.
Replaces	http://hdl.handle.net/1957/43717

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