Rate of catabolic pathways of glucose in the intact rat Public Deposited

http://ir.library.oregonstate.edu/concern/graduate_thesis_or_dissertations/cr56n4808

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  • Virtually nothing is known regarding the quantitative significance of the several catabolic sequences involved in the breakdown of glucose in the intact rat. Studies in this and other laboratories have shown that the Embden-Meyerhof-Parnas pathway, the pentose phosphate pathway and the glucuronic acid pathway are operative in several tissues of the rat. Work in other laboratories has revealed that the pentose cycle and presumably the glucuronic acid cycle operate to a minor extent in several tissues. In the present study, the primary pathways of glucose catabolism in the intact rat were identified and their relative participation evaluated. In order to evaluate the relative participation of the concurrent operation of the several catabolic pathways of glucose in the intact rat a novel method has been employed. This method, which is referred to as the catabolic rate method, is based upon high resolution radiorespirometry. The respiratory ¹⁴CO₂ was determined by means of an ion-chamber-electrometer assembly. Rats were infused continuously for a ten hour period with specifically labeled ¹⁴C-glucose substrates. Under these conditions a metabolic steady state with regard to glucose utilization was realized. Thus, data on the interval ¹⁴CO₂ yield allows one to calculate the catabolic rates of the individual glucose pathways directly. These rates have never been directly measured before. A cross comparison of the rates of individual pathways provide one with information on the relative participation of these glucose pathways, information which has heretofore been unavailable for any biological system. By the use of the catabolic rate method, it was concluded that in the intact rat, glucose is catabolized predominantly by way of the Embden-Meyerhof-Parnas pathway, to the extent of approximately 83%. The pentose phosphate and the glucuronic acid pathways play a lesser but significant role in the overall catabolism of glucose, accounting approximately for 11% and 6% respectively. Not only has valuable information been obtained on the fundamental role played by these catabolic sequences but in addition a method has been developed which will allow one to probe more deeply into regulatory mechanisms operative in the intact rat in such areas as biochemical toxicology, endocrinology and etc. This method should also find extensive application in the study of carbohydrate metabolism in health and disease.
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