Graduate Thesis Or Dissertation
 

Variants of infectious hematopoietic necrosis virus selected with glycoprotein-specific monoclonal antibodies

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https://ir.library.oregonstate.edu/concern/graduate_thesis_or_dissertations/cz30pw747

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  • The development of an effective infectious hematopoietic necrosis virus (IHNV) vaccine depends on a better understanding of the antigenic structure and variation among isolates. Three objectives to elucidate this were: (1) to generate a panel of antigenic derivatives of the IHNV glycoprotein by monoclonal antibody-mediated selection of neutralization-resistant variant viruses, (2) to determine if mutation in a critical site for viral neutralization affects viral virulence in vivo and characteristics in vitro, and (3) to establish an antigenic relationship between the Round Butte (RB) and 193-110 strains of IHNV. A single anti-glycoprotein monoclonal antibody, RB/B5, was used to select neutralization-resistant variants of the RB and 193-110 strains of IHNV. The virulence of the battery of IHNV variants was tested in rainbow trout by waterborne challenge. Two of these variants, RB-1 and 193-110-4. exhibited decreased virulence for the host. Vaccination with RB-1 and 193- 110-4 conferred protection to rainbow trout challenged with wild-type virus. Interference did not appear to be the mechanism for protection. In vitro, variants RB-1 and 193-110-4 retained several wild-type characteristics. Titers were normal. differences among the structural proteins were not detected. variants were not temperature-sensitive mutants. and variants were neutralized by hyperimmune serum. However, variant replication was slower than wild-type virus at permissive temperatures and as a result viral plaque size was smaller. Experiments with neutralizing and nonneutralizing monoclonal antibodies indicated that the RB and 193-110 strains have overlapping neutralization epitopes and share conserved sequences at a similar region on the IHNV glycoprotein.
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