Graduate Thesis Or Dissertation
 

Partial purification and properties of a columnaricin isolated from the myxobacterium Chondrococcus columnaris

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  • Of the 14 strains of Chondrococcus columnaris examined, 4 produced antagonistic substances that inhibited the growth of other strains of this species. The antagonistic substance produced by strain 3-P63-12 was examined in greater detail. Since the inhibitory substance had an extremely narrow spectrum of activity, and was not transmissible in series, it was concluded that the antagonistic substance was a bacteriocin (columnaricin). It was found that the columnaricin was produced spontaneously by broth cultures and that a maximal amount of activity was attained at the end of the logarithmic phase of growth. Partial purification of the columnaricin was achieved by chemical treatment, differential centrifugation, and column chromatography. Although two distinct active fractions were eluted from diethylaminoethyl(DEAE)-cellulose during the purification procedure, both fractions initially were found to have identical characteristics. The elution profile of the activity from a Bio-Gel P-10 column indicated that the columnaricin had a minimum molecular weight of 20,000. Other studies showed that the columnaricin was not sedimentable at 100,000 x g for 2 hours. It appeared that the columnaricin was a relatively low molecular weight protein, heat labile and completely inactivated in the presence of trypsin.
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