Studies on the fatty acid and lipid composition of Clostridium botulinum Public Deposited

http://ir.library.oregonstate.edu/concern/graduate_thesis_or_dissertations/d791sj66b

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  • Routine studies of bacterial fatty acid and lipid composition, made possible by recently developed techniques, have brought about an increasing interest in the physiological significance of these compounds. The purpose of this study was to characterize the fatty acids of the vegetative cells of Clostridium botulinum 33A, as the first step in an investigation of the possible relationship between the fatty acid content and radiation resistance of this organism. Lipids were extracted from lyophilized cells. Free fatty acids were separated by column chromatography. Methyl esters of the free fatty acids and the fatty acids from the other lipids were characterized by gas-liquid chromatography. The lipid extract was separated into classes using thin-layer chromatography. The results show that this organism possesses a complete fatty acid spectrum ranging from C₁₀ to C₂₀ with the exception of C₁₉. The great majority of the fatty acids present are straight-chain saturates or straight-chain monounsaturates. The lipids include large amounts of free fatty acids and phospholipids.
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