Graduate Thesis Or Dissertation

 

Morphology of epididymal spermatozoa from inbred and linecross bulls Public Deposited

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https://ir.library.oregonstate.edu/concern/graduate_thesis_or_dissertations/dr26z196t

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  • Data were presented on epididymal spermatozoa from 29 inbred and linecross bulls raised at Oregon State University in connection with the Western Regional Beef Cattle Breeding Project, W-1. The inbred bulls came from four inbred lines. Three of these inbred lines were Herfords and the other line was Angus. The 21 linecross bulls were produced from reciprocal crosses of the three Hereford lines. The bulls were put on test at 450 pounds. They were fed one part concentrate to two parts roughage up to 800 pounds at which time the concentrates were increased. They were slaughtered at 1000 pounds. The epididymides were stripped from the testes at the time of slaughter and sperm were obtained from the head and tail of the epididymides for making slides. An eosin-fast green differential stain was used to make slides for characterizing the spermatozoa as dead or alive and for detecting physiological abnormalities. Two slides were made from each location on the epididymis and labeled as head or tail and left or right. This made 16 slides per bull. The slides were then studied and 200 spermatozoa were counted on each slide. Only ten to 25 spermatozoa were counted in one spot in order to obtain an overall picture of each slide. The number of live, dead, normal, and types of abnormals were recorded. The different types of abnormals counted and recorded were neck beads, tail beads, tailless heads, coiled tails, and bent tails. An analysis of variance was run for breeding types, sides, and locations and all possible interactions between these variables. Statistical significance was determined by use of the F test. Means were calculated for breeding types and locations. To estimate general combining ability effects, means of lines I, II, or III combined with each of the other two lines are compared. To estimate specific combining ability, means of I x II, I x III, and II x III are compared. The analysis of variance indicated that there were statistically significant differences between breeding types for live spermatozoa, tail beads, and bent tails. Since a particular breeding type that was superior in one characteristic was also inferior in another, there was no generally superior breeding type.
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