In vitro detection and typing of toxins produced by Clostridium botulinum types A and B by immunoelectrophoretic techniques Public Deposited

http://ir.library.oregonstate.edu/concern/graduate_thesis_or_dissertations/dr26z215t

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  • Polyacrylamide gel disc electrophoresis of concentrated culture media which had supported growth of Clostridium botulinum types A and B was shown to be effective in separating the toxin molecules from hemagglutinating molecules. Since the hemagglutinins of the two types are immunologically identical and are present in toxin and toxoid preparations, available antitoxins cross-react. The electrophoretic separation and elution of the toxins is observed as a possible means for future preparation of non-cross-reacting antitoxins. The combination of a double-diffusion agar precipitin screening test applied to toxin-containing media and an immunoelectrophoretic toxin detection system demonstrates that the presence and identity of a botulinal toxin in a substrate can be determined in vitro. Future application of the immunoelectrophoretic system described depends on availability of more specific non-cross-reacting antitoxins of high quality.
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  • File scanned at 300 ppi using Capture Perfect 3.0 on a Canon DR-9050C in PDF format. CVista PdfCompressor 5.0 was used for pdf compression and textual OCR.
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  • description.provenance : Approved for entry into archive by Patricia Black(patricia.black@oregonstate.edu) on 2014-04-15T18:31:13Z (GMT) No. of bitstreams: 1 NiedermeyerGlennJ1968.pdf: 2054377 bytes, checksum: 4169592c4bcd5e14722494fb35b49825 (MD5)
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  • description.provenance : Made available in DSpace on 2014-04-16T17:08:33Z (GMT). No. of bitstreams: 1 NiedermeyerGlennJ1968.pdf: 2054377 bytes, checksum: 4169592c4bcd5e14722494fb35b49825 (MD5) Previous issue date: 1968-04-09

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